TOXICITY OF BACILLUS THURINGIENSIS CRY1-TYPE INSECTICIDAL TOXIN TO GEOGRAPHICALLY DISTANT POPULATIONS OF TOMATO PINWORM
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چکیده
منابع مشابه
The Bacillus thuringiensis insecticidal toxin binds biotin-containing proteins.
Brush border membrane vesicles from larvae of the tobacco hornworm, Manduca sexta, contain protein bands of 85 and 120 kDa which react directly with streptavidin conjugated to alkaline phosphatase. The binding could be prevented either by including 10 microM biotin in the reaction mixture or by prior incubation of the brush border membrane vesicles with an activated 60- to 65-kDa toxin from Bac...
متن کاملCloning, sequencing, and expression of the Bombyx mori receptor for Bacillus thuringiensis insecticidal CryIA(a) toxin.
Bacillus thuringiensis strains produce insect-specific Bt toxins. Bt CryIA(a) toxin binds to a 175-kDa glycoprotein (BtR175) on the microvillus membranes of columnar cells in the Bombyx mori midgut and causes lysis of the cells. BtR175 was purified, and its cDNA was cloned. The cDNA encodes a newly identified 193.3-kDa preproprotein form of BtR175 that includes nine extracellular cadherin repea...
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Most Bacillus thuringiensis strains producing toxins active on lepidoptera contain several plasmid-encoded delta-endotoxin genes and package related protoxins into a single inclusion. It was previously found that in B. thuringiensis subsp. aizawai HD133, which produces an inclusion comprising the CryIAb, CryIC, and CryID protoxins, there is a spontaneous loss in about 1% of the cells of a 45-mD...
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Genetically modified crops expressing insecticidal proteins from Bacillus thuringiensis (Bt) have dramatically increased in acreage since their introduction in the mid-1990’s. Although the insecticidal mechanisms of Bt target specific pests, concerns persist regarding direct and indirect effects on non-target organisms. In the field, spiders may be exposed to Bt toxins via multiple routes, incl...
متن کاملA quadruple-enterotoxin-deficient mutant of Bacillus thuringiensis remains insecticidal.
Bacillus thuringiensis is the leading biopesticide used to control insect pests worldwide. Although they have a long record of safe use, under certain conditions commercial strains of B. thuringiensis have the ability to produce numerous putative enterotoxins that have been associated with food poisoning attributed to Bacillus cereus. Therefore, we designed a strategy to delete the genes encodi...
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ژورنال
عنوان ژورنال: Florida Entomologist
سال: 2003
ISSN: 0015-4040,1938-5102
DOI: 10.1653/0015-4040(2003)086[0222:tobtci]2.0.co;2